ChromatographySeparating a mixture, and calculating Rf.BiologyRf is the ratio of the distance moved by the spot tothe distance moved by the solvent front.Rf = distance moved by spot ÷ distance moved by solventSpot 3 cm, front 12 cmRf = 0.25More soluble in the solventHigher RfTravels further.More attracted to the paperLower RfTHE COMMON MISTAKEStarting the pencil line below the solventThe origin must sit above the solvent levelIf the spots are submerged they dissolve into the solvent instead of travelling upthe paper.Rf is only comparable under identical conditions: same solvent, samepaper, same temperature.Chromatographylearnposters.com
Chromatography — printable life science wall chart from LearnPosters. Free vector PDF, US Letter and A4.

What’s on the Chromatography poster

The rule, 3 worked examples, and the mistake everyone makes.

Rf is only comparable under identical conditions: same solvent, same paper, same temperature.

Questions about the Chromatography poster

What’s on the Chromatography poster?
The rule, 3 worked examples, and the mistake everyone makes. Rf is the ratio of the distance moved by the spot to the distance moved by the solven…; Rf = distance moved by spot ÷ distance moved by solvent; Spot 3 cm, front 12 cm = Rf = 0.25; More soluble in the solvent = Higher Rf; More attracted to the paper = Lower Rf; Common mistake: Starting the pencil line below the solvent → The origin must sit abov…. Rf is only comparable under identical conditions: same solvent, same paper, same temperature.
Who is the Chromatography poster for?
Chromatography belongs to the Biology section rather than to a school year, because life science is not something one grade owns. Anyone learning biochemistry can pin it up — a beginner, a student mid-course, or someone revising years later.
When should you use the Chromatography poster?
Rf is always between 0 and 1, and is characteristic under fixed conditions. A wall chart earns its place by being glanceable from where the work is happening, so Chromatography belongs on the wall where that life science work actually happens, within glancing distance, rather than filed away.
What other posters go with Chromatography?
Amino Acids, ATP and Food Tests sit alongside Chromatography in the Biology section. Printed together they make a wall rather than a single sheet, which is how a reference set actually gets used.Amino AcidsATPFood Tests
Is the Chromatography poster free to download and print?
Yes. Chromatography downloads as a free PDF with no account, no email and no watermark, like everything else in the Biology section. Print as many copies as you like for a home, a classroom, a library or a tutoring group; reselling the file is the only thing the licence rules out.Read the licence
What size does the Chromatography poster print at?
Chromatography is a vector PDF laid out for US Letter, and prints on A4 with Fit to page — the same file, no separate download. Because every mark on it is drawn rather than photographed, it stays sharp enlarged to A3, A2 or A1 at a copy shop. Colour carries emphasis only, so a greyscale print of Chromatography loses nothing.Printing guide

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